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human cesc cell lines hela  (iCell Bioscience Inc)
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iCell Bioscience Inc human cesc cell lines hela
The expression of transforming growth factor α (TGFA) in different patients was analysed by bioinformatics and immunohistochemistry. (A) TGFA expression difference analysis results of 33 tumours based on TCGA database data. (B) Pan‐cancer analysis of paired samples based on TCGA database data. (C) Differential analysis of TGFA expression in unpaired cervical squamous cell carcinoma and endocervical adenocarcinoma <t>(CESC)</t> samples. (D) Differential analysis of TGFA expression in paired CESC samples. (E) TGFA expression differential analysis based on GSE6791 data. (F) TGFA expression differential analysis based on GSE7410 data. (G) Immunohistochemical results of TGFA in normal cervical tissues and cervical squamous cell carcinoma with different degrees of differentiation. (H) Immunohistochemical results of TGFA in normal and adenocarcinoma tissues of the cervix. (I) Group comparison of TGFA immunohistochemical results in 16 normal cervical tissues, 6 precancerous lesions (CIN‐III), and 42 CESC tissues. Significance identifier: ns (no significance), p ≥ 0.05; *, p < 0.05; ** p < 0.01; *** p < 0.001.
Human Cesc Cell Lines Hela, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The expression of transforming growth factor α (TGFA) in different patients was analysed by bioinformatics and immunohistochemistry. (A) TGFA expression difference analysis results of 33 tumours based on TCGA database data. (B) Pan‐cancer analysis of paired samples based on TCGA database data. (C) Differential analysis of TGFA expression in unpaired cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) samples. (D) Differential analysis of TGFA expression in paired CESC samples. (E) TGFA expression differential analysis based on GSE6791 data. (F) TGFA expression differential analysis based on GSE7410 data. (G) Immunohistochemical results of TGFA in normal cervical tissues and cervical squamous cell carcinoma with different degrees of differentiation. (H) Immunohistochemical results of TGFA in normal and adenocarcinoma tissues of the cervix. (I) Group comparison of TGFA immunohistochemical results in 16 normal cervical tissues, 6 precancerous lesions (CIN‐III), and 42 CESC tissues. Significance identifier: ns (no significance), p ≥ 0.05; *, p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: TGFA expression is associated with poor prognosis and promotes the development of cervical cancer

doi: 10.1111/jcmm.18086

Figure Lengend Snippet: The expression of transforming growth factor α (TGFA) in different patients was analysed by bioinformatics and immunohistochemistry. (A) TGFA expression difference analysis results of 33 tumours based on TCGA database data. (B) Pan‐cancer analysis of paired samples based on TCGA database data. (C) Differential analysis of TGFA expression in unpaired cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) samples. (D) Differential analysis of TGFA expression in paired CESC samples. (E) TGFA expression differential analysis based on GSE6791 data. (F) TGFA expression differential analysis based on GSE7410 data. (G) Immunohistochemical results of TGFA in normal cervical tissues and cervical squamous cell carcinoma with different degrees of differentiation. (H) Immunohistochemical results of TGFA in normal and adenocarcinoma tissues of the cervix. (I) Group comparison of TGFA immunohistochemical results in 16 normal cervical tissues, 6 precancerous lesions (CIN‐III), and 42 CESC tissues. Significance identifier: ns (no significance), p ≥ 0.05; *, p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: iCell Bioscience Inc., Shanghai, provided the human CESC cell lines HeLa and SiHa.

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Comparison

Influence of transforming growth factor α (TGFA) expression in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) on diagnosis and prognosis of patients. (A) TGFA diagnostic ROC curve; the area under the ROC curve is between 0.5 and 1. The closer the AUC is to 1, the better the diagnostic effect. When the AUC is between 0.5 and 0.7, the accuracy is low, when the AUC is between 0.7 and 0.9, the accuracy is moderate, and when the AUC is above 0.9, the accuracy is high. (B, C) Clinical significance of TGFA expression with age and menopausal status. (D–F) Stratified KM survival curves for overall survival (OS), disease‐specific survival (DSS), and progression‐free period (PFI) based on TGFA expression. (G) Binary logistic regression analysis results of correlation between TGFA expression level and clinical features in 58 patients. The data is incomplete because some records are missing. Significance identifier: ns (no significance), p ≥ 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: TGFA expression is associated with poor prognosis and promotes the development of cervical cancer

doi: 10.1111/jcmm.18086

Figure Lengend Snippet: Influence of transforming growth factor α (TGFA) expression in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) on diagnosis and prognosis of patients. (A) TGFA diagnostic ROC curve; the area under the ROC curve is between 0.5 and 1. The closer the AUC is to 1, the better the diagnostic effect. When the AUC is between 0.5 and 0.7, the accuracy is low, when the AUC is between 0.7 and 0.9, the accuracy is moderate, and when the AUC is above 0.9, the accuracy is high. (B, C) Clinical significance of TGFA expression with age and menopausal status. (D–F) Stratified KM survival curves for overall survival (OS), disease‐specific survival (DSS), and progression‐free period (PFI) based on TGFA expression. (G) Binary logistic regression analysis results of correlation between TGFA expression level and clinical features in 58 patients. The data is incomplete because some records are missing. Significance identifier: ns (no significance), p ≥ 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: iCell Bioscience Inc., Shanghai, provided the human CESC cell lines HeLa and SiHa.

Techniques: Expressing, Biomarker Discovery, Diagnostic Assay

Functional validation of TGFA in CESC cell lines. (A) Fluorescence maps of lentivirus transfected HeLa and SiHa cells. (B) HeLa and SiHa cells were transfected with shTGFA, and the expression level of TGFA was detected by qRT‐PCR. (C, D) The proliferation of HeLa and SiHa cells was detected by CCK‐8. (E–G) The ability of HeLa and SiHa cells to metastasize was examined by wound healing assay. Significance identifier: ns (no significance), p ≥ 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: TGFA expression is associated with poor prognosis and promotes the development of cervical cancer

doi: 10.1111/jcmm.18086

Figure Lengend Snippet: Functional validation of TGFA in CESC cell lines. (A) Fluorescence maps of lentivirus transfected HeLa and SiHa cells. (B) HeLa and SiHa cells were transfected with shTGFA, and the expression level of TGFA was detected by qRT‐PCR. (C, D) The proliferation of HeLa and SiHa cells was detected by CCK‐8. (E–G) The ability of HeLa and SiHa cells to metastasize was examined by wound healing assay. Significance identifier: ns (no significance), p ≥ 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: iCell Bioscience Inc., Shanghai, provided the human CESC cell lines HeLa and SiHa.

Techniques: Functional Assay, Biomarker Discovery, Fluorescence, Transfection, Expressing, Quantitative RT-PCR, CCK-8 Assay, Wound Healing Assay

Functional validation of TGFA in CESC cell lines. (A, C) The proliferation of HeLa and SiHa cells was detected by colony formation assay. (B, D) The invasion ability of HeLa and SiHa cells was detected by Transwell assay. (E–G) The expression of IL‐1A, IL‐17, MMP1, MMP10 and MMP13 after TGFA expression reduction was detected by western blot. Significance identifier: ns (no significance), p ≥ 0.05; *, p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: Journal of Cellular and Molecular Medicine

Article Title: TGFA expression is associated with poor prognosis and promotes the development of cervical cancer

doi: 10.1111/jcmm.18086

Figure Lengend Snippet: Functional validation of TGFA in CESC cell lines. (A, C) The proliferation of HeLa and SiHa cells was detected by colony formation assay. (B, D) The invasion ability of HeLa and SiHa cells was detected by Transwell assay. (E–G) The expression of IL‐1A, IL‐17, MMP1, MMP10 and MMP13 after TGFA expression reduction was detected by western blot. Significance identifier: ns (no significance), p ≥ 0.05; *, p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: iCell Bioscience Inc., Shanghai, provided the human CESC cell lines HeLa and SiHa.

Techniques: Functional Assay, Biomarker Discovery, Colony Assay, Transwell Assay, Expressing, Western Blot